The players who were optioned included pitchers Jose De Paula, Juan Pablo Oramas and Erik Hamren, outfielders Rymer Liriano, Blake Tekotte and infielder Edinson Rincon.
The two players who were reassigned to Minor League camp were left-handed pitcher Robbie Erlin and catcher Jason Hagerty.
The eight players will likely start playing in Minor League games beginning on Friday, said Padres manager Bud Black.
This is the second round of camp cuts, as the Padres reassigned five players to Minor League camp on Monday.
Rincon impressed during Cactus League play, going 7-for-10 with five doubles. Liriano, considered one of the Padres' top prospects, hit .400 (6-for-15). Tekotte, who made his Major League debut last season, hit .400 with one home run and five RBIs.