"It's a little concerning," said Padres manager Bud Black. "We don't know if it's a cramp, strain or a pull. We'll know more when this thing sets up in 24 hours."
Gyorko went 1-for-5 in the 10-inning loss to the Rockies, a game that saw the Padres lose a three-run lead in the ninth inning before Dexter Fowler's single won it in the 10th.
Gyorko is hitting .284 this season and leads all Major League rookies with 66 hits. He also leads the team in home runs (eight) and RBIs (25) in 60 games.
"Hopefully, it's just a cramp," he said. "It happened on the last step before I slid. When I got inside the dugout, it spasmed pretty bad."
If Gyorko misses any time -- the Padres open a series on Monday at Petco Park against the Braves -- the team could use utility man Alexi Amarista at second base, but they'd likely need to add another infielder to take Gyorko's spot on the active roster.
Corey Brock is a reporter for MLB.com. Keep track of @FollowThePadres on Twitter This story was not subject to the approval of Major League Baseball or its clubs.